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KMID : 1103720130680020175
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Journal of the Korean Society of Radiology
2013 Volume.68 No. 2 p.175 ~ p.185
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Comparison of Superparamagnetic Iron Oxide Labeling Efficiency between Poly-L-Lysine and Protamine Sulfate for Human Mesenchymal Stem Cells: Quantitative Analysis Using Multi-Echo T2* Magnetic Resonance Imaging
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Suh Ji-Yeon
Lee Jeong-Hyun
Lee Chang-Kyung
Shin Ji-Hoon
Choi Choong-Gon
Kim Jeong-Kon
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Abstract
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Purpose: To quantify in vitro labeling efficiency of protamine sulfate (PS) and poly-L-lysine (PLL) for labeling of human mesenchymal stem cells (hMSCs) with superparamagnetic iron oxide (SPIO) using multi-echo T2* magnetic resonance (MR) imaging at 4.7 T.
Materials and Methods: The hMSCs were incubated with SPIO-PS or SPIO-PLL complexes. Their effects on the cell metabolism and differentiation capability were evaluated, respectively. The decrease of iron concentrations in the labeled cells were assessed immediately, and at 4 d after labeling using multi-echo T2* MR imaging at 4.7 T. The results were compared with those of Prussian blue colorimetry.
Results: The hMSCs were labeled more efficiently by SPIO-PLL than SPIO-PS without any significant effect on cell metabolism and differentiation capabilities. It was feasible to quantify the iron concentrations in SPIO-agarose-phantoms and in agarose mixture with the labeled cells from T2* maps obtained from multi-echo T2* MRI. However, the iron concentration of the labeled cells was significantly higher by T2*-maps than the results of Prussian blue colorimetry.
Conclusion: The hMSCs can be effectively labeled with SPIO-PLL complexes more than with SPIO-PS without significant change in cell metabolism and differentiation. In vitro quantification of the iron concentrations of the labeled is feasible from multi-echo T2* MRI, but needs further investigation.
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KEYWORD
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Mesenchymal Stem Cell, Cellular Magnetic Resonance, Transfection Agent
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